Date of Award
2019
Document Type
Thesis
Degree Name
Bachelors
Department
Natural Sciences
First Advisor
Saarinen, Emily
Area of Concentration
Biology
Abstract
Millions of people are affected by mosquito-borne diseases, causing problems for people specifically in Sub-Saharan Africa. Scientists have started looking at control methods using microbes to better manage mosquitoes, primarily looking at organisms in the bacterial kingdom. In this project, I attempted to survey the fungal microbiome of three mosquito genera using ITS1 and ITS4 primers. Results from this experiment showed mostly mosquito DNA was amplified using these methods, however, this data has uncovered important information about biases which occur in the process of amplifying multiple genomes using ITS1 and ITS4 primers.
Recommended Citation
Teal, Evan Towers, "A novel approach to in situ microbiome surveys of fungi in mosquitoes" (2019). Theses & ETDs. 5817.
https://digitalcommons.ncf.edu/theses_etds/5817