The Search for MicroRNAs Encoded by the Influenza A Virus

Date of Award

2011

Document Type

Thesis

Degree Name

Bachelors

Department

Natural Sciences

First Advisor

Walstrom, Katherine

Keywords

MicroRNA, Gene Expression Regulation, Influenza Virus

Area of Concentration

Chemistry

Abstract

MicroRNAs are small non-coding regulatory RNA molecules. They regulate protein translation by binding to mRNAs preventing the mRNAs from being translated into proteins. MicroRNAs have been found in most eukaryotic cells and in DNA viruses such as the herpesviruses; they have not yet been found in RNA viruses. Influenza A is a RNA virus that causes a huge economic and health impact worldwide. This study identified one possible mature microRNA encoded by the 2009 Swine Flu strain of the Influenza A virus. Several pre-miRNA hairpins were predicted using a computational program designed to predict pre-miRNAs within viral genomes. Possible microRNAs within these hairpins were identified and examined using qPCR, DNA gel electrophoresis, and sequencing. One possible mature microRNA was identified that was in the proper location on a predicted pre-miRNA hairpin and was the proper length to be a mature miRNA.

Rights

This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

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