Investigations into UV/Ozone Patterned Photolithography and Vesicle Adsorption Kenetics of Liquid-Crystalline POPC Novel Techniques to Determine Structure and Dynamics of Lipid Bilayers

Date of Award

2004

Document Type

Thesis

Degree Name

Bachelors

Department

Natural Sciences

First Advisor

Johal, Malkait

Keywords

Lipid bilayers, Imaging Ellipsometry, Surface Tension

Area of Concentration

Chemistry

Abstract

In this body of research, two new techniques are outlined for the investigation into fluid lipid bilayer systems. The first technique uses imaging ellipsometry as a new, noninvasive method for measuring in situ kinetics of selective bilayer formation in UV/Ozone patterned lipid bilayers. This method allows for high resolution and real-time monitoring of topographic contrast. The ellipsometry data obtained was correlated with the well established technique of fluorescence microscopy. The second technique uses a surface tensiometer, which also acts as a sensitive force balance, to obtain information about vesicle adsorption onto hydrophilic Si surfaces. We show that an irreversibly bound inner leaflet of POPC remains bound to the hydrophic surface and that subsequent bilayer formation on the inner leaflet is possible. Force and contact angle data from the tensiometer allow investigations into surface dynamics which occur on POPC- Si systems.

Rights

This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

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