Author

Sarah Wilhide

Date of Award

2020

Document Type

Thesis

Degree Name

Bachelors

Department

Natural Sciences

First Advisor

Gilchrist, Sandra

Area of Concentration

Biology

Abstract

In this present time, most scientists agree that we are living through the sixth mass extinction and that this event is partly due to humans’ effect on the environment (Abatzoglou and Williams, 2016; Payne et al., 2016). Maintaining biodiversity is a key component in mitigating the many effects of anthropogenic climate change. Biodiversity must first be evaluated to determine if and what conservation steps should be taken. Monitoring biodiversity across the various scale of life is an undertaking and traditional monitoring practices are not always sufficient. The use of environmental DNA and subsequent analysis may aid in this endeavor. Environmental DNA, or eDNA, is extraorganismal and collected from soil, water, and air and can be multiplied with PCR. Collected DNA can be digitally compared with the DNA of known species to establish identity through a process called metabarcoding. The use of eDNA as a conservation tool is promising for monitoring biodiversity as it is inexpensive, fast, and noninvasive. This thesis aims to discuss the benefits and failings of eDNA as a conservation tool, as well as discuss practical applications and the direction future research may take this technology.

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