Date of Award
2018
Document Type
Thesis
Degree Name
Bachelors
Department
Natural Sciences
First Advisor
Walstrom, Katherine
Area of Concentration
Biochemistry
Abstract
Many aspects have not yet been completely understood about rha-1 in C. elegans. In this thesis study, we attempted to build a CRISPR-Cas9 targeting vector to fuse GFP to the C-terminus of the rha-1 gene with the aim to acquire broader knowledge of the expression and functions of rha-1 in C. elegans. The CRISPR-Cas9 targeting vectors tagged by GFP at the C-terminus of the rha-1 gene were constructed using multiple DNA assembly tools such as: SapTrap Assembly, Gibson Assembly and Hi-Fi Assembly. Together, the obtained findings confirmed that the attempts to generate these CRISPR-Cas9 targeting vectors were unsuccessful due to numerous possible reasons. As a result, future work should primarily focus on developing an alternative way to tackle the inefficiency of constructing CRISPR-Cas targeting vectors for the rha-1 gene.
Recommended Citation
Fawwaz, Najla F., "MUTATING RNA HELICASE A USING CRISPR-CAS9 METHODS IN C. ELEGANS" (2018). Theses & ETDs. 5510.
https://digitalcommons.ncf.edu/theses_etds/5510