THE EFFECTS OF MATRIX METALLOPROTEINASE-9 ON CAL-27 TUMOR INVASION IN VITRO
Date of Award
2016
Document Type
Thesis
Degree Name
Bachelors
Department
Natural Sciences
First Advisor
Gilchrist, Sandra
Area of Concentration
Natural Sciences
Abstract
Research has shown that there is increased presence of myeloid derived suppressor cells in human tumors of various cancers and that granulytic crd11 MDSC cells show a high expression of matrix metalloproteinase 9 (MMP9). MMP9, a matrixin, has been shown to have an effect on tumor invasion, angiogenesis, and metastasis. Its proteolytic qualities suggest that it aids in invasion through degradation of the extracellular matrix (ECM). A series of in vitro chamber invasion assays were used to duplicate and simplify the tumor microenvironment to determine if invasion of Cal-27 (adenosquamous carcinoma cell line) cancer cells could be facilitated through degradation of the ECM by MMP9. Results from this experiment indicate that MMP9 does not have an effect on the ECM in vitro without the presence of an activator. Further studies should test its effects on Cal-27 proliferation before using a chamber invasion assay to test for its role in tumor invasion.
Recommended Citation
Lee, Cornell, "THE EFFECTS OF MATRIX METALLOPROTEINASE-9 ON CAL-27 TUMOR INVASION IN VITRO" (2016). Theses & ETDs. 5236.
https://digitalcommons.ncf.edu/theses_etds/5236