Incorporation of Active Lactate Dehydrogenase into Polyelectrolyte Multilayer Assemblies
Date of Award
2005
Document Type
Thesis
Degree Name
Bachelors
Department
Natural Sciences
First Advisor
Johal, Malkait
Keywords
Lactate Dehydrogenase, Polyelectrolyte Multilayers, Electrostatic Self-Assembly
Area of Concentration
Chemistry
Abstract
The goal of this research is to assemble novel polyelectrolyte thin film systems comprised of a polyanion, a polycation and a biologically active enzyme, lactate dehydrogenase (LDH). The function of this catalytic device will be monitored as the conversion of pyruvate to lactate is catalyzed by LDH. This work investigates the immobilization, catalytic activity, and reusability of LDH in polyelectrolyte multilayer film assemblies constructed via electrostatic self-assembly and spin-assembly. Enzymes, and other biomolecules, have been successfully integrated into polyelectrolyte multilayer assemblies in the past but little attention has been given to investigating conditions which prevent leaching of the enzyme out of the film assemblies into aqueous solution. Results presented in this thesis demonstrate that electrostatically bound LDH between polycation layers of poly(ethylenimine) (PEI) remains catalytically active without leaching into solution. This has significant application as a model system for biosensors or surface catalytic devices.
Recommended Citation
Martin, Rebecca, "Incorporation of Active Lactate Dehydrogenase into Polyelectrolyte Multilayer Assemblies" (2005). Theses & ETDs. 3546.
https://digitalcommons.ncf.edu/theses_etds/3546
Rights
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.