Site-Directed Mutagenesis of a Single-Nucleotide Mutation in Familial Atrial Fibrillation
Date of Award
2003
Document Type
Thesis
Degree Name
Bachelors
Department
Natural Sciences
First Advisor
Walstrom, Katherine
Keywords
Atrial Fibrillation, Overlap Extension Mutagenesis, Polymerase Chain Reaction
Area of Concentration
Biology
Abstract
Atrial fibrillation is the most common form of cardiac arrhythmias with a morbidity of 0.3% to 0.4% in the adult population. The majority of the cases of AF are related to cardiovascular abnormalities. Idiopathic atrial fibrillation is defined as the specific kind of atrial fibrillation without organic or structural heart diseases. The hereditary pattern of this familial form of the disease has been characterized as autosomal dominant. Clinical evaluations and genomic analysis of a Chinese kindred with familial idiopathic AF has identified KvLQT1, a gene ericoding a voltage-sated potassium channel in human cardiac myocytes on chromosome 11, as the potential disease gene. This paper describes the site-directed mutagenesis procedures used to produce mutated KvLQTl-containing plasmids for future electrophysiological characterization in Xenopus oocytes. Alterations in the electrophysiological properties of the mutated ion channels can be used to confirm the molecular pathogenesis of atrial fibrillation.
Recommended Citation
Zhuang, Hanyi, "Site-Directed Mutagenesis of a Single-Nucleotide Mutation in Familial Atrial Fibrillation" (2003). Theses & ETDs. 3337.
https://digitalcommons.ncf.edu/theses_etds/3337
Rights
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